61 research outputs found
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Enabling community-based metrology for wood-degrading fungi
Background: Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on single-cell organisms and extensive ancillary equipment to produce precursors for downstream manufacturing processes. Alternative forms of bioproduction based on solid-state fermentation and wood-degrading fungi could enable more direct means of manufacture. However, basic methods for cultivating wood-degrading fungi are often ad hoc and not readily reproducible. Here, we developed standard reference strains, substrates, measurements, and methods sufficient to begin to enable reliable reuse of mycological materials and products in simple laboratory settings.
Results: We show that a widely-available and globally-regularized consumer product (Pringles™) can support the growth of wood-degrading fungi, and that growth on Pringles™-broth can be correlated with growth on media made from a fully-traceable and compositionally characterized substrate (National Institute of Standards and Technology Reference Material 8492 Eastern Cottonwood Whole Biomass Feedstock). We also establish a Relative Extension Unit (REU) framework that is designed to reduce variation in quantification of radial growth measurements. So enabled, we demonstrate that five laboratories were able to compare measurements of wood-fungus performance via a simple radial extension growth rate assay, and that our REU-based approach reduced variation in reported measurements by up to ~ 75%.
Conclusions: Reliable reuse of materials, measures, and methods is necessary to enable distributed bioproduction processes that can be adopted at all scales, from local to industrial. Our community-based measurement methods incentivize practitioners to coordinate the reuse of standard materials, methods, strains, and to share information supporting work with wood-degrading fungi
Modeling the Large Scale Structures of Astrophysical Jets in the Magnetically Dominated Limit
We suggest a new approach that could be used for modeling both the large
scale behavior of astrophysical jets and the magnetically dominated explosions
in astrophysics. We describe a method for modeling the injection of magnetic
fields and their subsequent evolution in a regime where the free energy is
magnetically dominated. The injected magnetic fields, along with their
associated currents, have both poloidal and toroidal components, and they are
not force free. The dynamic expansion driven by the Lorentz force of the
injected fields is studied using 3-dimensional ideal magnetohydrodynamic
simulations. The generic behavior of magnetic field expansion, the interactions
with the background medium, and the dependence on various parameters are
investigated.Comment: Accepted to ApJ, May 10, 2006 issue, 12 figures total (3 color
figures
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Synthetic genome recoding: new genetic codes for new features.
Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construction that includes DNA synthesis, assembly by recombination, and troubleshooting, is now comparable to costs of early stage development of drugs or other high-tech products. Here, we discuss several recently published assembly methods and provide some thoughts on the future, including how synthetic efforts might benefit from the analysis of natural recoding processes and organisms that use alternative genetic codes
Enabling community-based metrology for wood-degrading fungi
Background: Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on single-cell organisms and extensive ancillary equipment to produce precursors for downstream manufacturing processes. Alternative forms of bioproduction based on solid-state fermentation and wood-degrading fungi could enable more direct means of manufacture. However, basic methods for cultivating wood-degrading fungi are often ad hoc and not readily reproducible. Here, we developed standard reference strains, substrates, measurements, and methods sufficient to begin to enable reliable reuse of mycological materials and products in simple laboratory settings.
Results: We show that a widely-available and globally-regularized consumer product (Pringles™) can support the growth of wood-degrading fungi, and that growth on Pringles™-broth can be correlated with growth on media made from a fully-traceable and compositionally characterized substrate (National Institute of Standards and Technology Reference Material 8492 Eastern Cottonwood Whole Biomass Feedstock). We also establish a Relative Extension Unit (REU) framework that is designed to reduce variation in quantification of radial growth measurements. So enabled, we demonstrate that five laboratories were able to compare measurements of wood-fungus performance via a simple radial extension growth rate assay, and that our REU-based approach reduced variation in reported measurements by up to ~ 75%.
Conclusions: Reliable reuse of materials, measures, and methods is necessary to enable distributed bioproduction processes that can be adopted at all scales, from local to industrial. Our community-based measurement methods incentivize practitioners to coordinate the reuse of standard materials, methods, strains, and to share information supporting work with wood-degrading fungi
High Magnetic Shear Gain in a Liquid Sodium Stable Couette Flow Experiment; A Prelude to an alpha-Omega Dynamo
The -phase of the liquid sodium - dynamo experiment
at NMIMT in cooperation with LANL has successfully demonstrated the production
of a high toroidal field, from the radial
component of an applied poloidal magnetic field, . This enhanced toroidal
field is produced by rotational shear in stable Couette flow within liquid
sodium at . The small turbulence in stable Taylor-Couette flow
is caused by Ekman flow where . This high
-gain in low turbulence flow contrasts with a smaller -gain in
higher turbulence, Helmholtz-unstable shear flows. This result supports the
ansatz that large scale astrophysical magnetic fields are created within
semi-coherent large scale motions in which turbulence plays only a smaller
diffusive role that enables magnetic flux linkage.Comment: 5 pages, 5 figures, submitted PRL revised version: add one author,
minor typo'
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Enhancing crop growth in rooftop farms by repurposing CO2 from human respiration inside buildings
Peer reviewed: TrueAcknowledgements: We would like to thank Sidney Hare and Tony Pham for their assistance collecting samples. The entire community at the Boston University Academy, especially Paige Brewster, Travis Bain, and Cynthia Taylor, also were essential in carrying out this project. This content also appeared in the PhD thesis titled Enhancing plant growth and carbon harvesting for sustainable agriculture (Buckley, 2020).Integrating cities with the surrounding environment by incorporating green spaces in creative ways would help counter climate change. We propose a rooftop farm system called BIG GRO where air enriched with carbon dioxide (CO2) produced through respiration from indoor spaces is applied through existing ventilation systems to produce a fertilization effect and increased plant growth. CO2 measurements were taken inside 20 classrooms and at two exhaust vents on a rooftop at Boston University in Boston, MA. Exhausted air was directed toward spinach and corn and plant biomass and leaf number were analyzed. High concentrations of CO2 persisted inside classrooms and at rooftop exhaust vents in correlation with expected human occupancy. CO2 levels averaged 1,070 and 830 parts per million (ppm), reaching a maximum of 4,470 and 1,300 ppm CO2 indoors and at exhaust vents, respectively. The biomass of spinach grown next to exhaust air increased fourfold compared to plants grown next to a control fan applying atmospheric air. High wind speed from fans decreased growth by approximately twofold. The biomass of corn, a C4 plant, experienced a two to threefold increase, indicating that alternative environmental factors, such as temperature, likely contribute to growth enhancement. Enhancing growth in rooftop farms using indoor air would help increase yield and help crops survive harsh conditions, which would make their installation in cities more feasible.</jats:p
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